A family of doublestranded RNA viruses, some of which (Reovirus) previously were included with Echoviruses, and others (Orbivirus), with arboviruses Virions are 6080 nm in diameter, usually naked, and ether resistant;Each type was found to contain singlestranded adeninerich (Arich) RNA in addition to DS RNA The isolation and properties of this singlestranded RNA are described Materials and MethodsReovirus types 1 (Lang), 2 (D5 Jones), and 3 (Abney) were obtained from the American Type Culture Collection Polyadenylic acid (poly A) and adenylate oligonucleotide of chain lengthOSTIGOV Journal Article Distinct binding sites for zinc and doublestranded RNA in the reovirus outer capsid protein sigma3 Distinct binding sites for zinc and doublestranded RNA in the reovirus outer capsid protein sigma3 Full Record;
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Double stranded rna is found in reovirus
Double stranded rna is found in reovirus- A likely candidate for the role of such molecules is double stranded RNA (dsRNA) A similar approach has been previously used in our laboratory for the purification of dsRNA of a Drosophila reoviruslike virus with a segmented genome (Pasyukova and Mukha, 09) To remove the cells and cell debris from the semen, we used two different approaches In the first approach, The reovirus core has also interested Harrison's lab because it may bear similarities to other doublestranded RNA viruses that are important pathogens In upcoming studies, Harrison's group will be looking at the relationship between the structure and function of the outer shell proteins that get reoviruses and rotaviruses into cells
Structure Of The Reovirus Outer Capsid And Dsrna Binding Protein S3 At 1 8 A Resolution The Embo Journal
The virions of reoviruses (the name is a shortening of respiratory enteric orphan viruses) lack an outer envelope, appear spheroidal, measure about 70 nanometres (nm;1 nm = 10 9 metre) across, have two icosahedral capsids, and contain a core of segmented, doublestranded RNAReoviruses are known to contain doublestranded (DS) RNA' It was therefore surprising to find that RNA extracted from highly purified reovirus type 3 and analyzed without further fractionation had a purine/pyrimidine ratio of 15 and an adenylic acid content of more than 40 moles per cent In an effort to understand the basis for this observation, the RNA's of all three reovirus serotypes
Grass carp reovirus (GCRV), a fatal pathogen to aquatic animals, can provoke severe hemorrhagic disease in grass carp and causes a mortality rate of up to 85% during the summer months, when water temperatures range between 24°C and 30°C 1, 2GCRV infects grass carp, Ctenopharyngodon idellus, was found to be capable of infecting black carp MylopharyngodonUnique in that they possess a segmented doublestranded (ds) RNA genome Using reovirus as a model member of this family, we have developed the only functional reverse genetics system for a member of this family with ten or more genome segments Using this system, we have previously identified the flanking 5' sequences required by an engineered s2 ssRNA for efficient Doublestranded RNA virus outer shell assembly by (Aquareovirus) 27, mammalian reovirus (Orthoreovirus) 28 , blue tongue virus (Orbivirus) 29,30, rice
Capsids are of icosohedral symmetry with two layers of capsomeresWatanabe & Graham, 1967) Three classes of messenger RNA of reovirusinfected cells were found which, under proper conditions, hybridize — Howard Hughes Medical Institute researchers have solved the structure of an important component of the reovirus, a doublestranded RNA virus that bears similarity to pathogens
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OSTIGOV Journal Article Nature of the polypeptide encoded by each of the 10 doublestranded RNA segments of reovirus type 3 Nature of the polypeptide encoded by each of the 10 doublestranded RNA segments of reovirus type 3 Full Record;Abstract Cycloheximide, at a concentration of 10 μg/ml, rapidly blocked protein synthesis in L cells infected with reovirus When the drug was added before 5 hr postinfection, synthesis of both single and doublestranded varieties of virusspecific ribonucleic acid (RNA), which normally commences between 6 and 7 hr after infection, was blockedThe doublestranded (ds)RNA viruses represent a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve), and virion organization (Tnumber, capsid layers, or turrets) Members of this fascinating group include the rotaviruses, renowned globally as the commonest cause of gastroenteritis in young children,
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Mammalian reoviruses are nonenveloped viruses that contain a genome of 10 doublestranded RNA segments (reviewed in reference 24) The doublestranded RNA molecules are classified by size into three large (L), three medium (M), and four small (S) gene segments There are three reovirus serotypes, which can be differentiated by the capacity of antireovirus The CP's of BMV and RRV are capable of binding to the doublestranded S4 RNA in an electrophoretic mobility shift assay In the context of a viral infection, however, we note it is likely that fully assembled virions may not result in activation of TLR3 signaling if the dsRNAs are not exposed We have added highly purified BMV virions and Reovirus virions to BEAS2B cells It's what's known as singlestranded RNA (ssRNA), and it can be found throughout the natural world Less common in nature is doublestranded RNA (dsRNA), which has two strands and resembles the wellknown DNA double helix It's found in some viruses, but for the past few decades, people have been developing synthetic dsRNA for a range of
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Reoviruses are nonenveloped, doublestranded RNA viruses with icosahedral symmetry and three capsid layers Chapter 7 Viral zoonoses The Duke researchers hypothesized that the binding of normal reoviruses to a host cell's membrane might make that cell more vulnerable to infection by naked, genetically altered reovirus RNAThe doublestranded (ds)RNA viruses represent a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve), and virion organization (Tnumber, capsid layers, or turrets) Members of this fascinating group include the rotaviruses, renowned globally as the commonest cause of gastroenteritis in young children,A corresponding trimodal distribution of double stranded RNA was found by sucrose densitygradient centrifugation and poly acrylamide gel electrophoresis whether the RNA was derived from purified virus or infected tissue (Bellamy et al, 1967;
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By atomic absorption analysis, we determined that the reovirus outer capsid protein sigma 3, which binds doublestranded RNA (dsRNA), is a zinc metalloprotein Using Northwestern blots and a novel zinc blotting technique, we localized the zinc and dsRNAbinding activities of sigma 3 to distinct V8 proteasegenerated fragments Zincbinding activity was contained within an aminoReovirus RNAtranscriptase is an integral component of the maturevirus andis carried into the host cell during infection1 2 Theintimate association of the reovirus doublestranded RNAgenome with the virion tran scriptase providesanefficient mechanism forthein viito transcription of early mRNAwithout the need for host cell polymerases or virusinduced enzymes' 68 segmented, doublestranded RNA genome that infect a variety of mammalian species 69 but cause disease only in the very young Reovirus is transmitted via the fecaloral route 70 and disseminates from the intestine to virtually all organs via hematogenous or neural 71 routes Infection of newborn mice with serotype 3 reovirus strains causes neuronal 72 apoptosis,
Frontiers Double Stranded Rna In Human Seminal Plasma Genetics
Reoviridae Sciencedirect
Differential Delivery of Genomic DoubleStranded RNA Causes Reovirus StrainSpecific Differences in Interferon Regulatory Factor 3 Activation Serotype 3 (T3) reoviruses induce substantially more type 1 interferon (IFNI) secretion than serotype 1 (T1) strainsA series of 94 isolates of reovirus from humans, cattle, and mice, showed extensive variability in the patterns of migration of the ten doublestranded RNA genome segments This variation was found in all three serotypes, and involved all ten genome segments, including the segment responsible for serological specificity Although a single pattern was present among severalReovirus definition, any large virus of the family Reoviridae, having doublestranded RNA and a polyhedral capsid, including those causing infantile gastroenteritis See more
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Igor Tamm found that reovirus has doublestranded RNA as its genome (25) The peculiarity of reovirus was underscored by the demonstration later that an RNA polymerase in the virion of reovirus is able to a symmetrically transcribe the doublestranded RNA (26) Th is was the first virionbound RNA dependent RNA polymerase ever found and followed after the finding of theIn brief, singlestranded (plusstranded, ss) and/or doublestranded (ds) RNA of reovirus serotype 3 (ST3 virus) is lipofected into L929 mouse fibroblasts together with a rabbit reticulocyte lysate in which ss or melted dsRNA has been translated After 8 hr the cells are then infected with a helper virus, ST2 reovirus2 days ago Less common in nature is doublestranded RNA (dsRNA), which has two strands and resembles the wellknown DNA double helix It's found in some viruses, but for the past few decades, people have
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Retroviruses Double Stranded Rna Viruses Boundless Microbiology
The genes of the Dearing strain of reovirus serotype 3, which consist of doublestranded RNA, have been cloned into pBR322 by tailing both strands of each gene with poly(A), transcribing them with reverse transcriptase, selfhybridizing the cognate plus and minus cDNA strands, incubating them with Escherichia coli DNA polymerase I to ensure that they are complete, and cloning the doubleReoviruses Any of a group of doublestranded RNA viruses which occur in the respiratory and digestive tracts of healthy individuals but which may cause infantile diarrhoea (rotaviruses) and Colorado tick fever (orbiviruses) A partacronym from 'Respiratory', 'Enteric', 'Orphan' Differential Delivery of Genomic DoubleStranded RNA Causes Reovirus StrainSpecific Differences in Interferon Regulatory Factor 3 Activation Stuart JD (1) (2), Holm GH (3), Boehme KW (4) (2)
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Reoviruses Rotaviruses Caliciviruses
1 day ago Less common in nature is doublestranded RNA (dsRNA), which has two strands and resembles the wellknown DNA double helix It's found in some viruses, but for the past few decades, people haveThose with a genome made of several pieces of doublestranded RNA Example reovirus Retroviruses Their RNA (also singlestranded) is copied by reverse transcriptase into aGonzalezLopez et al, 03) The structure of the mammalian reovirus core has been solved by Xray crystallography (Reinisch et al, 00) In this structure, the structural
A Plasmid Based Reverse Genetics System For Animal Double Stranded Rna Viruses Cell Host Microbe
Disruption Of Specific Rna Rna Interactions In A Double Stranded Rna Virus Inhibits Genome Packaging And Virus Infectivity
Doublestranded RNA viruses are classified in two separate phyla Duplornaviricota and Pisuviricota (specifically class Duplopiviricetes), which are in the kingdom Orthornavirae and realm Riboviria The two groups do not share a common dsRNA virus ancestor Doublestranded RNA viruses evolved two separate times from positivestrand RNA viruses The genome of reovirus comprises at least 10 segments of dsRNA that vary in length, including long (39 kbp), medium (22 kbp), and short (12 kbp) RNA segments Kato et al show that RIGI and MDA5 recognize the short and long dsRNA segments of the reovirus genome, respectively In the case of EMCV infection, the authors show that the viral genome forms longN pl re·o·vi·rus·es Any of a family of viruses that contain doublestranded RNA and are associated with various diseases in animals, including human respiratory and gastrointestinal infections r (espiratory) e (nteric) o (rphan) virus American Heritage® Dictionary of the English Language, Fifth Edition
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The S2 gene nucleotide sequences of prototype strains of the three reovirus serotypes were determined to gain insight into the structure and function of the S2 translation product, virion core protein σ2 The S2 sequences of the type 1 Lang, type 2 Jones, and type 3 Dearing strains are 1,331 nucleotides in length and contain a single large open reading frame that could encode a proteinIn addition, it has also been implicated in the resistance of the virus to interferon by binding doublestranded RNA and thus inhibiting the doublestranded RNAdependent protein kinase (MartinezCostas et al, 00;This RNA is less dense than viral RNA and resists digestion by ribonuclease It is therefore, considered to be a doublestrandedform of poliovirus RNAAsignificant amount of this material is found in association with a membranous virusspecific particulate The recent demonstration of the natural occurrence of doublestranded RNA in reovirus
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Reovirus Structure And Infectious Cycle A Reovirus Is A Download Scientific Diagram
Reovirus doublestranded RNA genomes and polyIC induce downregulation of hypoxiainducible factor 1α Author Hotani, Takuma, Tachibana, Masashi, Mizuguchi, Hiroyuki, Sakurai, Fuminori Source Biochemical and biophysical research communications 15 v460 pp ISSN X Subject Reoviridae, antineoplastic activity, cathepsins, cytoplasm, doublestranded RNAGenomes contain doublestranded, segmented RNA (MW 1016 × 10 6 );Definition of reovirus any of a family (Reoviridae) of doublestranded RNA viruses that have a virion with icosahedral structural symmetry but may appear spherical, that have a capsid with one to three concentric protein layers, and that include many plant or animal pathogens (such as the rotaviruses and the causative agent of bluetongue)
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The interaction between the helicase DHX33 and IPS1 as a novel pathway to sense doublestranded RNA and RNA viruses in myeloid dendritic cells Cell Mol Immunol 11, 49–57 (14) https//doiReovirus sigma3 is a virion outer shell protein that also binds dsRNA and stimulates translation by blocking activation of the dsRNAdependent protein kinase, PKR Purified sigma3 was shown by gel shift assay to bind specifically to RNA duplexes of minimal length 3245 base pairs PKR binding to dsRNA was prevented by sigma3, and translation inhibition of luciferase reporter by PKR This is also called positivestranded RNA Examples poliovirus;
Cell Entry Independent Role For The Reovirus M1 Protein In Regulating Necroptosis And The Accumulation Of Viral Gene Products Biorxiv
Reovirus Nonstructural Protein Sns Acts As An Rna Stability Factor Promoting Viral Genome Replication Journal Of Virology
Nontoxic concentrations of polycations stimulate binding of reovirus doublestranded (ds) RNA to the macrophages by forming polycationdsRNA complexes either in the medium or at the cell surface These complexes enter the cell in endocytic vacuoles and are concentrated in secondary lysosomes Despite exposure to the acid hydrolases within this cell compartment, the dsRNA andIn the course of reovirus replication the doublestranded virion RNA serves as a template for reovirus mRNA ( strand) formation The reovirus mRNA in turn serves as a template for the synthesis of the complementary () strand The latter remains associated with the template, thus forming doublestranded virion RNA The virion associated enzymes can be activated in vitro to synthesize reovirusNo doublestranded RNA has been found free within virusinfected cells other than that in newly sylnthesized virus and subviral particles The virus and sub viral particles appear to be associated with virussynthesizing factories MaterialsVirus Dearing strain of reovirus 3, cloned,1 was used as stock virus after two passages Cell culture L cells, strain 929, were grown in suspension
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Transmission Electron Micrograph Tem Reveals Some Of The Morphologic Details Displayed By The Irituia Strain Of The Changuinola Virus A Member Of The Genus Orbiviruses Which Is One Of Nine Genera Of
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